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Literature summary extracted from

  • Wille, I.; Rek, A.; Krenn, E.; Kungl, A.J.
    Biophysical investigation of human heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A: a mutual effect of enzyme oligomerisation and glycosaminoglycan ligand binding (2007), Biochim. Biophys. Acta, 1774, 1470-1476.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.30 expressed in Escherichia coli strain BL21(DE3)pLysS Homo sapiens
2.8.2.30 expression of the N-terminally His6-tagged truncated mutant isozyme 3-OST-3A lacking the cytoplasmic, transmembrane and SPLAG domain Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.8.2.30 additional information construction of a truncated version of 3-OST-3A starting at amino acid position G148, thereby lacking the cytoplasmic, transmembrane and SPLAG domain Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.30 33000
-
 x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE Homo sapiens
2.8.2.30 33743
-
 x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.30 heparan sulfate + 3'-phosphoadenylyl sulfate Homo sapiens i.e. PAPS 3-O-sulfated heparan sulfate + adenosine 3',5'-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.30 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.30 Co2+ affinity chromatography and heparin-agarose column chromatography Homo sapiens
2.8.2.30 recombinant N-terminally His6-tagged truncated mutant isozyme 3-OST-3A lacking the cytoplasmic, transmembrane and SPLAG domain from Escherichia coli by cobalt and heparin affinity chromatography, followed by dialysis, to over 95% puritiy Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.2.30 addition of 6 M guanidinium-HCl results in a red shift of the emission maximum by 12 nm indicating that the enzyme is properly folded after purification Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.30 3'-phosphoadenylyl sulfate + chondroitin sulfate chondroitin sulfate exhibits similar high affinity binding to 3-OST-3A compared to heparan sulfate Homo sapiens ?
-
 ?
2.8.2.30 3'-phosphoadenylyl sulfate + heparin heparin exhibits similar high affinity binding to 3-OST-3A compared to heparan sulfate Homo sapiens ?
-
 ?
2.8.2.30 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine 3-OST-3A generates a unique heparan sulfate sequence that is predicted to specifically bind envelope glycoprotein D of Herpes Simplex virus type 1, but not antithrombin III Homo sapiens adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
 ?
2.8.2.30 heparan sulfate + 3'-phosphoadenylyl sulfate i.e. PAPS Homo sapiens 3-O-sulfated heparan sulfate + adenosine 3',5'-bisphosphate
-
 ?
2.8.2.30 additional information binding to different glycosaminoglycans, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.30 ? x * 33743, His6-tagged truncated mutant, amino acid sequence calculation, x * 33000, recombinant His6-tagged truncated mutant, SDS-PAGE Homo sapiens
2.8.2.30 More analysis of the overall structural integrity of recombinant 3-OST-3A by steady-state fluorescence spectroscopy, secondary structure with a content of 38.7% alpha-helix and 13.3% beta-sheet, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.8.2.30 3-O-sulfotransferase-3A
-
 Homo sapiens
2.8.2.30 3-OST-3A
-
 Homo sapiens
2.8.2.30 heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A
-
 Homo sapiens
2.8.2.30 heparan sulfate D-glucosaminyl 3-O-sulfotransferase-3A the enzyme generates a binding site for the envelope glycoprotein D of herpes simplex virus 1 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.30 additional information
-
 thermal unfolding kinetics of the purified recombinant His6-tagged truncated mutant protein, overview Homo sapiens